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Image Search Results
Journal: Molecular & Cellular Proteomics : MCP
Article Title: Characterization of Usher Syndrome Type 2-Associated Proteins in the Retina via Affinity Purification-Mass Spectrometry
doi: 10.1016/j.mcpro.2026.101526
Figure Lengend Snippet: Localization and coimmunoprecipitation of high-confidence USH2-interacting proteins in mouse photoreceptors . A – D , localization of COL6A1 ( A ), HEXIM1 ( B ), SIPA1 ( C ), and PTPN23 ( D ) in mouse photoreceptors relative to ADGRV1. For each panel, the right side shows single-channel images corresponding to the boxed region in the merged images. For COL6A1 and HEXIM1, intensity profile plots were generated along a line intersecting the ADGRV1 signal at the three labeled positions. OS, outer segment; CC, connecting cilium; IS, inner segment; ONL, outer nuclear layer. The scale bars represent 5 μm. E , representative immunoblots showing that SIPA1 coimmunoprecipitates with usherin and that EIF2B4 coimmunoprecipitates with ADGRV1. ADGRV1, adhesion G protein–coupled receptor V1; COL6A1, collagen type VI alpha 1 chain; HEXIM1, hexamethylene bis-acetamide-inducible protein 1; PTPN23, protein tyrosine phosphatase non-receptor type 23; SIPA1, signal-induced proliferation-associated 1.
Article Snippet:
Techniques: Generated, Labeling, Western Blot
Journal: World Journal of Gastroenterology
Article Title: Fas counterattack in cholangiocarcinoma: A mechanism for immune evasion in human hilar cholangiocarcinomas
doi: 10.3748/wjg.v7.i6.860
Figure Lengend Snippet: FasL positive in human hilar cholangiocarcinom as (brown). SABC × 200
Article Snippet: Detection of FasL expression and CD45 positive cells was performed using a
Techniques:
Journal: World Journal of Gastroenterology
Article Title: Fas counterattack in cholangiocarcinoma: A mechanism for immune evasion in human hilar cholangiocarcinomas
doi: 10.3748/wjg.v7.i6.860
Figure Lengend Snippet: Expression of FasL in cholangiocarcinoma cell line. QBC939 × 200
Article Snippet: Detection of FasL expression and CD45 positive cells was performed using a
Techniques: Expressing
Journal: World Journal of Gastroenterology
Article Title: Fas counterattack in cholangiocarcinoma: A mechanism for immune evasion in human hilar cholangiocarcinomas
doi: 10.3748/wjg.v7.i6.860
Figure Lengend Snippet: Expression of FasL mRNA in human cholangiocarcinoma cells QBC939. M: DL 2000 Marker; 1: FasL; 2: FasL+β-actin
Article Snippet: Detection of FasL expression and CD45 positive cells was performed using a
Techniques: Expressing, Marker
Journal: World Journal of Gastroenterology
Article Title: Fas counterattack in cholangiocarcinoma: A mechanism for immune evasion in human hilar cholangiocarcinomas
doi: 10.3748/wjg.v7.i6.860
Figure Lengend Snippet: Western blotting of FasL protein with mAb from QBC939 cell cultures clone 33 from QBC939 cell cultures
Article Snippet: Detection of FasL expression and CD45 positive cells was performed using a
Techniques: Western Blot
Journal: bioRxiv
Article Title: S100a4 + alveolar macrophages accelerate the progression of precancerous atypical adenomatous hyperplasia by promoting fatty acid metabolism
doi: 10.1101/2024.10.17.618873
Figure Lengend Snippet: S100a4-OE MH-S promoted malignant transformation of MLE12 epithelial cells in vitro. A. S100a4 mRNA expression level in MH-S after plasmid transfection. B. S100a4 protein expression level in MH-S after plasmid transfection. C. CCK8 assay showing cell proliferation of MLE12 after coculture with S100a4-OE MH-S. D. Colony forming ability of MLE12 after coculture, as shown by colony formation assay. E. Cell cycle distribution of MLE12 after coculture, as shown by cell cycle analysis. F. Wound healing assay showing cell migration of MLE12 after coculture. G. Intracellular ROS level of MLE12 after coculture. H. Transmission electron microscopy showing the morphological changes of mitochondria in MLE12 after coculture. I. Western blotting of DNA damage marker p-γH2ax, EMT markers (E-cadherin, N-cadherin, and Vimentin), and stem-like markers (Cd44 and Cd133) in MLE12 after coculture. J. Western blotting of tumorigenesis associated proteins (c-Myc, p-Erk, Sftpa, Vegfa, and Hif-1α) in MLE12 after coculture. K. Western blotting of macrophage pro-tumor indicators (Vegfa, Mmp9, Tgf-β, and Hif-1α) in S100a4-OE MH-S. L. Western blotting of fatty acid metabolism-related proteins (Cpt1a and Acot2) and angiogenesis-related proteins (Anxa2 and Ramp1) in S100a4-OE MH-S, and angiogenesis-related proteins (Anxa2 and Ramp1) in MLE12 after coculture. OE: overexpression; NC: negative control; ROS: reactive oxygen species; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001
Article Snippet: Immunoblotting was performed with the following primary antibodies: S100a4 (Abcam), β-actin (Cell Signaling Technology),
Techniques: Transformation Assay, In Vitro, Expressing, Plasmid Preparation, Transfection, CCK-8 Assay, Colony Assay, Cell Cycle Assay, Wound Healing Assay, Migration, Transmission Assay, Electron Microscopy, Western Blot, Marker, Over Expression, Negative Control